Determining Genome Size from Spores of Seedless Vascular Plants

Determining Genome Size from Spores of Seedless Vascular Plants

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Seedless vascular plants, including ferns and lycophytes, produce spores to initiate the gametophyte stage and to complete sexual reproduction.Approximately 10% of them are apomictic through the production g5210t-p90 of genomic unreduced spores.Being able to measure the spore nuclear DNA content is therefore important to infer their reproduction mode.Here we present a protocol of spore flow cytometry that allows an efficient determination transpharm online shopping of the reproductive modes of seedless vascular plants.